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DNA双链断裂（double strand break，DSB）是极为严重的DNA损伤形式。如果不对其进行修复或修复错误及修复不及时，可导致基因组突变和更大范围的结构重排，引起细胞癌变或者基因组不稳定性相关疾病等。
HydroShear DNA剪切仪（图）
DNA片段仪 DNA 剪切仪 基因组测序
2023/4/18
HydroShearDNA片段仪是软件自动控制的，利用水力剪切的专业DNA片段仪。它为随机短 DNA片段的获得提供了最简便，重复性佳，控制灵活的自动操作平台，保证了下游文库构建等实验用 DNA 样本的质量。GeneMachines HydroShearDNA片段仪有多种剪切组件可选以获得不同大小的剪切片段，满足用户多种要求，其可获得的最小片段长度为1kb，且90%以上的片段在2倍片段大小范围内。剪...
据最新一期《自然·生物技术》发表的一项研究，在CRISPR基因编辑系统的基础上，美国麻省理工学院研究人员设计了一种新工具，可以更安全、更高效的方式剪除有缺陷的基因并用新基因替换它们。
重离子辐射引起的线粒体DNA突变定量分析
线粒体DNA 4 977大片段缺失 D310区点突变
2014/7/16
对重离子辐射引起的线粒体DNA突变进行定量分析。方法 使用X射线和重离子束对 人乳腺癌细胞MCF-7进行辐照，对照后细胞进行克隆存活分析；用real-time PCR定量检测线粒体DNA 4 977缺失，克隆测序法定量检测线粒体D310区突变。结果 克隆存活结果和辐照后D310多态性分布 显示重离子射线对MCF-7细胞的增殖抑制效果比X射线更为明显，引起的D310突变也更多，并且这些 突变体能在辐...
A Novel Non Radioactive PCR-DNA Probe for the Detection of Aflatoxin Producing Aspergillus Species from Major Food Crops Grown in India
AFB1-Aflatoxin B1 HPLC-High Performance Liquid Chromatography
2013/1/29
In the present study, a novel non radioactive digoxigenen labelled PCR-DNA probe was developed targeting nor-1 gene to assess the contamination of aflatoxigenic Aspergillus species in food grain sampl...
A New Biomarker for Hepatocellular Damage: Plasma Cell-Free DNA
Hepatocellular Damage Cell-Free DNA
2013/1/28
Background: Accumulating evidence has suggested that cell-free DNA (cf-DNA) enters the circulation following cell apoptosis or necrosis. An increased level of cf-DNA fragments has been found in the bl...
Rapid Method for Isolation of PCR Amplifiable Genomic DNA of Ralstonia solanacearum Infested in Potato Tubers
Ralstonia solanacearum PCR Detection Potato DNA
2013/1/28
The aim of the present study was to develop a very fast and simple genomic DNA isolation method for Ralstonia solanacearum which infest potato tubers. One hundred potato tubers were collected and ten ...
Epitope tagging has been widely
used for studies of protein structure and
function (8). The epitope tagging
process generally begins with a cDNA
encoding the protein of interest. A major
weakness...
Development of a microfluidic module for DNA purification from bacterial lysates via phenol extraction
DNA Purification Phenol Extraction Multi-Phase Microfluidics
2009/12/24
In this work microfluidic platforms designed to miniaturize the phenol extraction
technique commonly used to purify DNA from cell lysates have been developed.
First, a long serpentine device was use...
采用DNA和蛋白质吸收带的Kubelka-Munk光谱函数对离体的人正常胃、未分化胃腺癌、胃鳞状细胞癌和低分化胃腺癌的上皮组织进行鉴别诊断。实验采用带积分球附件的分光光度计获取组织的漫反射光谱。结果表明：在250～650 nm，人胃的上皮组织的癌变导致未分化胃腺癌、胃鳞状细胞癌和低分化胃腺癌等的上皮组织的Kubelka-Munk光谱函数f(r∞)及其对数log[f(r∞)]的平均值在DNA吸收带(...
DNase γ-dependent and -independent apoptotic DNA fragmentations in Ramos Burkitt's lymphoma cell line
DNase γ-dependent -independent apoptotic DNA
2009/12/16
DNA fragmentation is a biochemical hallmark of apoptosis. Several endonucleases, including CAD/DFF40 and endonuclease G, are implicated in DNA fragmentation. DNase γ has also been considered to be one...
Genetic Studies of Bone Diseases: Evidence for Involvement of DNA Damage Response Proteins in Bone Remodeling
p53 Atm c-Abl osterix osteoblast osteoporosis
2009/4/20
Bone remodeling is carried out by bone marrow mesenchymal stem cell derived osteoblasts, which form the bones, and hematopoeitic stem cell derived osteoclasts, which absorb the bones. Their actions a...
Phospholipid-Nucleic Acid Complexation: Biomolecular Energetics of DNA-Mg2+-Phosphatidylcholine Ternary Complex Formation, Compaction and Relevance as Lipoplex Formulation
polyelectrolyte phospholipid - polynucleotide nanostructures differential scanning microcalorimetry thermotropic phase behaviour non-viral gene delivery
2009/4/20
Thermodynamic features related to preparation and use of self-assemblies formed between multilamellar and unilamellar zwitterionic liposomes and polynucleotides with various conformation and sizes are...
Detection of Varicella-Zoster Virus DNA in the Iris of a Zoster Sine Herpete Patient
Zoster Sine Herpete polymerase chain reaction iris iridocyclitis
2009/4/20
A 55-year-old man presented with unilateral iridocyclitis and elevated intraocular pressure (IOP) in his right eye. Varicella-zoster virus (VZV) DNA was detected by polymerase chain reaction (PCR) in ...
Analysis of metal ion binding to DNA in regulating metallothionein-IIA gene expression
metallothionein metal responsive elements metal ions binding DNA bending
2009/4/1
The mammalian metallothioneins (MT) are effectively induced in response to the various heavy metal ions. The inductions of these genes are mainly based on the transcriptional activation and the metal ...