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Copper Binding of Nuclear RNA- and DNA-Binding Proteins p54nrb/NonO and PSF
copper p54nrb/Non-POU-domain-containing octamer binding protein (p54nrb/NonO) polypyrimidine tract-binding protein-associated splicing factor (PSF) nucleus
2009/3/23
The biological function of nuclear copper in mammalian cells is poorly understood because little is known about specific binding protein(s) for nuclear copper. We recently purified that p54nrb/Non-POU...
Mechanisms of the DNA Damage in Rat Kidney after Au Injection
Gold (Au) Copper (Cu) 8-hydroxydeoxyguanosine (8-OHdG) DNA damage Metallothionein (MT)
2009/3/16
Au (gold) is a heavy metal and its complexes are one of the more effective medicaments available for rheumatoid arthritis, although many authors have reported serious kidney damage during the chrysoth...
Brca2 C-terminus interacts with Rad51 and contributes to nuclear focus formation in double-strand break repair of DNA
Brca2 C-terminus interacts Rad51 double-strand break repair of DNA
2009/3/3
In humans and mice, the interaction between the breast cancer susceptibility protein, BRCA2, and RAD51 recombinase is essential for DNA repair by homologous recombination, the failure of this process ...
Evidence for inclusion of a segment of Escherichia coli genomic DNA in bovine tooth germ mRNA encoding salivary proline-rich protein P-B
Escherichia coli genomic DNA germ mRNA salivary proline-rich protein P-B
2009/3/3
In the course of cloning of bovine cDNA for proline-rich protein (PRP) P-B from bovine tooth germ cDNA, we found that one clone with 662 bp contained a 5'-terminal 393 bp (1-393 bp) sequence essenti...
DNA microarray analysis of changes in gene expression induced by 1,25-dihydroxyvitamin D3 in human promyelocytic leukemia HL-60 cells
DNA microarray analysis gene expression 1,25-dihydroxyvitamin D3 promyelocytic leukemia HL-60 cells
2009/3/2
Using a DNA microarray, we analyzed about 16,600 genes for changes in expression associated with the differentiation of human promyelocytic leukemia HL-60 cells induced by 1α,25-dihydroxyvitamin D3 ...
Extremely narrow nanopores developed in Professor Amit Meller’s laboratory (BME) may be just the tool scientists need to reach the goal of a fast and inexpensive method of sequencing entire genomes.Re...
Development of a diffusion limited microfluidic module for DNA purification via phenol extraction
diffusion limited microfluidic module DNA purification phenol extraction
2009/12/24
Purification of Deoxyribonucleic acid (DNA) by organicaqueous liquid extraction, also called phenol extraction, is a standard technique commonly utilized in biology laboratories.
In order to minimize...
南京医科大学专家尝试提取DNA“复活”千年女尸
DNA 复活 千年女尸
2008/10/10
美国好莱坞电影《侏罗纪公园》创造了一种大胆科学设想：一只蚊子吸取了恐龙的血液，恰巧又被琥珀完美保存至今日，科学家因此获取了恐龙的DNA，再现了恐龙时代。再大胆地设想一下，我们能不能让一个西汉人站在我们面前呢？南京医科大学生物医学专家日前进行的一项鲜为人知的有趣尝试，表明这种可能性既需高超的科技也要大自然巧妙的配合。不过，南医大的王林涛老师告诉记者：“在一具连云港女尸中，我们尚未能提取出她完整的DN...
Sequence-Specific Purification of DNA Oligomers in Hydrophobic Interaction Chromatography using Peptide Nucleic Acid Amphiphiles: Extended Dynamic Range
hydrophobic interaction chromatography DNA separation peptide nucleic acid affinity separation
2009/12/22
Sequence-Specific Purification of DNA Oligomers in Hydrophobic Interaction Chromatography using Peptide Nucleic Acid Amphiphiles: Extended Dynamic Range.
Effect of Electrostatic Interactions on Binding and Retention of DNA Oligomers to PNA Liposomes Assessed by FRET Measurements
Peptide nucleic acid(PNA) Liposome DNA adsorption Biosensors FRET
2009/12/22
The FRET signal was maintained in the presence of 10-,20-,40-,
and 60-mer DNA inhighionic-strength buffer,showing that the driving force for the desorption is electrostatic repulsion between the boun...
Sequence-Specific Binding of DNA to Liposomes Containing di-Alkyl Peptide Nucleic Acid (PNA) Amphiphiles
Sequence-Specific Binding DNA Liposomes Containing di-Alkyl Peptide Nucleic Acid
2009/12/22
Sequence-Specific Binding of DNA to Liposomes Containing di-Alkyl Peptide Nucleic Acid (PNA) Amphiphiles.